Screening of Low-temperature Degrading Corn Stalk Fungi by Illumina High-throughput Sequencing Technology

There are abundant cellulose degrading bacteria in corn straw and rhizosphere soil. The purpose of this study was to analyze the fungal community structure and the screening of low temperature degrading fungi. Corn straw and rhizosphere soil were collected. The community structure of fungi in corn straw and rhizosphere soil were analyzed by high-throughput sequencing. Carboxymethyl cellulose sodium was used as the sole carbon source to screen low-temperature cellulose degrading fungi and identified them by its sequencing. The results showed that 20715913 BP was obtained from the sequencing of corn straw and its root soil,and 82250 gene fragments were spliced,with an average length of 249.29 bp. There were five fungal portal were found,and the dominant genus was identified as Guehomyce. Through screening at 15 ℃,comparing the ratio of transparent circle produced by Congo red staining to colony diameter at 0,5,10 and 15 ℃,three cellulose degrading fungi with strong enzyme producing ability were FB1,FB7 and FB8. The degradation rate was 27.66%,21.53% and 23.01% respectively. Finally,it was determined that FB1 had the best cellulase secretion and degradation effect,and it was identified as Cladosporium pseudocladosporioides.