Purification of Polysaccharide from Mori Cortex by Macroporous Resin

In order to explore the macroporous resin suitable for separating and purifying mulberry bark polysaccharides, the optimal purification process parameters were studied. Methods:Through the static adsorption-elution, the adsorption capacity, adsorption rate and desorption rate of ten different types of macroporous resins (H103, HP20, AB-8, X-5, D-101, DM301, DA-201, NKA-9, HPD-722, HPD300) were investigated, and the best purified resin was preferred, then the effects of sample solution pH, concentration of sample, speed of sample, volume fraction of eluent, dosage of eluent and flow rate of elution on the purification process were studied to determine the best purification process parameters. Results:The macroporous resin D-101 was the best, the optimal sample loading conditions were:pH=3.0, sample loading concentration 4.0 mg/mL, sample loading speed 2.0 BV/h. The optimal elution conditions were:75% ethanol eluent, eluent dosage of 3.5 BV, flow rate of 1.0 BV/h. After the process, the purity of polysaccharide in Mori Cortex increased from 16.12%±1.20% to 74.45%±1.15%. Conclusion:The results showed that D-101 macroporous resin can enrich and purify polysaccharides in Mori Cortex, which would provide a theoretical basis for more efficient utilization of Mori Cortex resources.