Contents Determination of Syringoside, Ginsenoside Rg₁, Re and Rb₁ in Shuangcishen Capsule

High performance liquid chromatography (HPLC) method was established for the content determination of syringoside, ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁ in Shuangcishen capsules. Using Elite APS and Nano-Micro UniSil 5-120 C₁₈ as chromatographic columns, acetonitrile-water was used as mobile phase for gradient elution at a flow rate of 1.0 mL/min with sample volume of 10 μL, the contents of ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁ were detected at wavelength of 203 nm with column temperature of 35℃, while syringoside was detected at wavelength of 265 nm with column temperature of 30℃. The results showed that ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ and syringoside showed a good linear relationship in the range of 15.30~306.00 μg/mL (R²=0.9999), 16.95~339.00 μg/mL (R²=1.0000), 18.45~369.00 μg/mL (R²=1.0000) and 4.60~46.00 μg/mL (R²=0.9999). The detection limits were 0.77, 0.89, 0.93, and 0.12 μg/mL, and the limits of quantification were 2.21, 2.71, 2.74, and 0.38 μg/mL, respectively. The RSD values of the precision tests were 1.30%, 1.21%, 1.99%, and 0.79%. The RSD values of their repeatability tests were 2.49% and 2.35%, 2.55%, 0.80%. The average sample recovery rates were 100.13%, 100.50%, 102.33%, and 101.55%, and their RSD values were 0.67%, 0.99%, 0.29%, and 0.68%, respectively. The results also indicated that the sample solution was stable within 48 h. The method had high specificity and good reproducibility which would be applied for the determination of syringin and ginsenosides Rg₁, ginsenosides Re, and ginsenosides Rb₁ in Shuangcishen capsules. The research work would provide the basic data for the establishment of quality standards for Shuangcishen capsules.