Scavenging Effect of Honeysuckle and Dandelion Extracts on the Biofilm of Pseudomonas Stains from Meat

The scavenging effect of honeysuckle and dandelion extracts on the biofilm of Pseudomonas strains was researched in this study. The optimum culture temperature, time and fixing method of Pseudomonas were determined by single factor tests. The effects of honeysuckle extract and dandelion extract on the biofilm of Pseudomonas were evaluated by crystal violet method combined with scanning electron microscopy. The results showed that, Pseudomonas strains from meat had strong biofilm-forming ability with the highest value under 30℃ at 24 h. The scavenging effect of honeysuckle extract and dandelion extract on biofilm increased gradually with the increase of mass concentration (P<0.05). The honeysuckle extract and dandelion extracts, adding at 0 h had better scavenging effects on forming biofilm than adding at 24 h (P<0.05). The laser-scanning confocal microscope imaging results showed that, extracts scavenged biofilm by inbibiting bacterial growth above MIC by destroying the spatial structure of biofilm. With the extracts concentration was higher than MIC, the holes on mature biofilm were smaller and fewer. Extracts destroyed the structure of biofilm and lead to the formation of holes on the biofilm when lower than MIC. This study proved that honeysuckle and dandelion extracts had the effect of scavenging Pseudomonas biofilm with potential application value in meat preservation.