WANG Ying-zi, YAN Jian-yong, ZHANG Shi-wei. Detection of Triamcinolone Acetonide Indirect Competitive ELISA Based on Heterology Coating Strategy[J]. Science and Technology of Food Industry, 2020, 41(8): 263-267. DOI: 10.13386/j.issn1002-0306.2020.08.041
Citation: WANG Ying-zi, YAN Jian-yong, ZHANG Shi-wei. Detection of Triamcinolone Acetonide Indirect Competitive ELISA Based on Heterology Coating Strategy[J]. Science and Technology of Food Industry, 2020, 41(8): 263-267. DOI: 10.13386/j.issn1002-0306.2020.08.041

Detection of Triamcinolone Acetonide Indirect Competitive ELISA Based on Heterology Coating Strategy

More Information
  • Received Date: July 24, 2019
  • Available Online: November 12, 2020
  • An indirect competitive ELISA for triamcinolone acetonide(TCA)was developed to monitor TCA abuse. To obtain the TCA complete antigen,TCA hapten and keyhole limpet hemocyanin(KLH)were coupled by using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride(EDC)and N-Hydroxysuccinimide(NHS). The homologous detection antigen TCA-bovine serum albumin(TCA-BSA)and heterologous detection antigen Dexamethasone-BSA(DEX-BSA)were synthesized in the same way. After immunization and cell fusion,one strain of hybridoma cell line that could steadily secrete anti-TCA monoclonal antibody was prepared and its antibody(ascites)was prepared and purified. By using the antibody and two different detection antigens,both homologous and heterologous strategy indirect competitive enzyme linked immunosorbent assay(ic-ELISA)standard curve for detection of TCA was built,recoveries of TCA added to liquid milk was detected by the heterologous strategy. The results showed that the IC50 of homologous strategy ic-ELISA was 5.4 ng/mL,the IC50 of heterologous strategy ic-ELISA was 0.53 ng/mL.The method did not cross reacted with other glucocorticoids except halcinonide,budesonide and amcinonide. The recovery rates of TCA added to animal tissues ranged from 75% to 90% using 20% methanol/water. The method can be applied in TCA fast screening in animal tissue.
  • Related Articles

    [1]HUA Mei, FAN Meiling, LU Jiaxi, DONG Lina, SUN Yinshi. Study on the in Vitro Antioxidant and Bile Salts Binding Activity of the Dietary Fibers from Seven Edible Rraditional Chinese Medicines[J]. Science and Technology of Food Industry, 2021, 42(11): 314-320. DOI: 10.13386/j.issn1002-0306.2020070159
    [2]WANG Yong-gang, JIA Wen-ting, LIU Zhan-xia, YANG Hui. Optimization of Enzymatic Extraction of Dietary Fiber from Zizyphus jujube and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2020, 41(18): 176-181,186. DOI: 10.13386/j.issn1002-0306.2020.18.028
    [3]XIE Hui, HAN Ya-ting, SHAO Pei-lan, ZHANG Li-fen, ZHENG An-ran. Study on the antioxidant activity of soluble dietary fiber from jujube[J]. Science and Technology of Food Industry, 2017, (22): 37-41. DOI: 10.13386/j.issn1002-0306.2017.22.008
    [4]WANG jian-chen, PENG Kai-le, ZHANG Luan, ZHENG Shi-yao, ZHAO Yu-hong. Preparation and properties analysis of Lonicera wine residue antioxidant dietary fiber yogurt[J]. Science and Technology of Food Industry, 2016, (23): 227-232. DOI: 10.13386/j.issn1002-0306.2016.23.034
    [5]GONG Si-si, TANG Xiao-ming, ZHENG Jun, ZHAO Qiang, XIONG Hua. Composition analysis and antioxidant activities of okara soluble dietary fiber prepared by a variety of enzymes[J]. Science and Technology of Food Industry, 2016, (16): 117-121. DOI: 10.13386/j.issn1002-0306.2016.16.015
    [6]CHEN Hai-hong, YIN Peng-fei, YIN Jun-yi, XIE Ming-yong. Physicochemical properties and antioxidant activities of water soluble dietary fiber from Ganoderma atrum[J]. Science and Technology of Food Industry, 2016, (08): 116-119. DOI: 10.13386/j.issn1002-0306.2016.08.015
    [7]QIAN Hai-feng, HUANG Dong-yun, YUAN Hua-ning, ZHANG Hui, WANG Li. Effects of cellulase enzyme modification on soluble dietary fibre content and antioxidant activity of rice bran[J]. Science and Technology of Food Industry, 2014, (15): 112-115. DOI: 10.13386/j.issn1002-0306.2014.15.014
    [8]LIU Wan, MA Hai-le, HUANG Liu-rong. Study on the modification of dietary fiber from garlic skin and antioxidantion[J]. Science and Technology of Food Industry, 2014, (12): 172-175. DOI: 10.13386/j.issn1002-0306.2014.12.028
    [9]茶多酚对色拉油的抗氧化作用[J]. Science and Technology of Food Industry, 1999, (06): 27-28. DOI: 10.13386/j.issn1002-0306.1999.06.069
    [10]柿叶乙醇提取物在猪油中的抗氧化性研究[J]. Science and Technology of Food Industry, 1999, (05): 22-23. DOI: 10.13386/j.issn1002-0306.1999.05.006
  • Cited by

    Periodical cited type(5)

    1. 陈酉,王素娟,张飞宇,杨林林,乔璐,董诚明,董宁. 基于斑马鱼模型的丹参花中酚酮类成分美白及抗氧化效果探究. 时珍国医国药. 2024(08): 1868-1871 .
    2. 何维彬,高亮,艾玉廷,周建富,龚行楚,杨振中,蒋剑平. 羊栖菜化学成分及药理活性研究进展. 中草药. 2023(07): 2327-2337 .
    3. 冯敏,幸宏伟,尤琳烽,刘小娟. 洛神花花青素提取工艺及抗氧化活性研究. 重庆工商大学学报(自然科学版). 2023(06): 1-7 .
    4. 匡婷,方宗穆,张名位,张瑞芬,池建伟,黄菲,贾栩超,董丽红,马勤,邓梅,马永轩,郝娟,赵东. 羊栖菜腥味物质分析及其脱除方法比较. 现代食品科技. 2022(11): 285-297 .
    5. 梁美娜,吴明江,陈胜,苏来金. 羊栖菜多糖的提取纯化及功能活性研究进展. 食品安全质量检测学报. 2021(21): 8305-8312 .

    Other cited types(3)

Catalog

    Article Metrics

    Article views (274) PDF downloads (9) Cited by(8)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return