Inhibitory Activity of Chlorogenic Acid on the Quorum Sensing of Pseudomonas fluorescens and its Spoilage Activity

Firstly,the inhibitory activity of quorum sensing of chlorogenic acid was determined by the detection model of Chromobacterium violaceum CV026 strain,and the effect of chlorogenic acid on the signal molecules released by Pseudomonas fluorescens was detected by HPLC-MS/MS. Based on the evaluation indexes of extracellular enzyme activity,ferritin,colony and swimming,the regulation of chlorogenic acid on colony induction activity and putrefaction of Pseudomonas fluorescens were analyzed by adding exogenous signal molecules.The results showed that chlorogenic acid could reduce the AHLs released by Pseudomonas fluorescens. Chlorogenic acid could effectively inhibit siderophore production,extracellular protease and lipase,swarming and swimming motility of Pseudomonas fluorescens. The concentration was positively correlated with its inhibitory effect. These results showed that chlorogenic acid had a great inhibitory activity,this study provided a basis for expanding the industrial application of chlorogenic acid as a novel inhibitor of spoilage bacteria.