Optimization of Ultrasonic Enzymatic Extraction of Procyanidins from Garcinia mangostana L.Pericarp

The ultrasonic enzymatic extraction of procyanidins from Garcinia mangostana L. pericarp was optimized. On the basis of single factor experiments,enzyme dosage,enzymolysis time,enzymolysis temperature,ultrasound power and ultrasound time were selected as independent variables,the yield of procyanidins was used as the response value. The three factors and three level response surface tests were designed by Box-Behnken experiment to carry out regression analysis and optimize the extraction process parameters. The influence factors on the yield of procyanidins were as follows:Enzymolysis time > enzymolysis temperature > ultrasound power. The results showed that the ultrasonic enzymatic extraction conditions of optimization by response surface method were as follows:Enzyme dosage 2%,enzymolysis time 68 min,enzymolysis temperature 58.8℃,ultrasound power 320 W,ultrasound time 20 min. The actual yield was 12.29%,which had 1.68% relative error with the predicted optimum yield 12.50%. The results of this study would provide a scientific basis for the comprehensive utilization of mangosteen pericarp.