Optimization for Cellulase-microwave Extraction Process and Its Chemical Composition of Flavonoids Substance from Adinandra nitida Merr.ex H.L.Li

The extraction yield of flavonoids was taken as the index,based on the single factor experiments,Box-Behnken response surface methodology was used to optimize the cellulase-microwave extraction process of flavonoids from Adinandra nitida Merr.ex H.L.Li with the extraction yield of flavonoids as the index. The cell wall microstructure of the extraction were observed by scanning electron microscope(SEM)and the composition of flavonoids from Adinandra nitida Merr.ex H.L.Li were analyzed by high performance liquid chromatography quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS). Results showed that the optimal extraction were:Extraction with 50% ethanol at a material/liquid ratio of 1:40 g/mL,pH4.0 with an enzyme dose of 0.08%,cellulase hydrolysis for 60 min at 51℃,followed by the assistance of microwave treatment for 3 min at 450 W power. Under these conditions,the average yield of flavonoids from Adinandra nitida Merr.ex H.L.Li. was 35.01%±0.75%,which was 66.87% and 18.12% higher than that of cellulase and microwave treatment alone. The cell wall of Adinandra nitida Merr.ex H.L.Li was broken more thoroughly than that of cellulase or microwave treatment alone,which was favorable for dissolution of components. 13 components obtained by optimized extraction were detected through HPLC-Q-TOF-MS analysis,including catechin,procyanidin,epicatechin,luteoloside,rhoifolin,camellianin B,quercitroside,camellianin A,apigenin,chrysoeriol,rosamultin,palmitic acid and ursolic acid. This study would provide a preliminary basis for the development and utilization of Adinandra nitida Merr.ex H.L.Li.