Optimization of the High Voltage Pulsed Electric Field Wall Breaking Technology of Ganoderma Spores Powder by Response Surface Method

In this experiment,the wall breaking test of ganoderma spores was carried out by using high voltage pulsed electric field to improve its wall breaking rate,the single factor test was conducted with electric field intensity,pulse width and pulse number as test factors. Response surface tests were carried out based on single experiment.The results showed that the effects degree of the three factors on the wall breaking of ganoderma spores powder were as follows:electric field intensity,pulse width and pulse number. The optimum process parameters for breaking the wall of ganoderma spores powder were obtained as follows:The electric field intensity 25 kV/cm,the pulse width 80 μs,and the pulse number 40000,the rate of wall breaking reached 66.74%±0.03%. The polysaccharides and total triterpenes of ganoderma spores powder were(0.99±0.11) g/100 g and(1.79±0.03) g/100 g,respectively.