Optimization of Extraction Process and Characterization Analysis of Collagen from Camel Palm

The camel paw as raw material,extraction time,enzyme content,acetic acid concentration and the ratio of material to liquid were used as experimental factors to optimize the extraction process of collagen extracted from camel paw by pepsin,and the properties of collagen from camel paw were analyzed. The results showed that the optimum extraction process of collagen from camel palm was extraction time 48 h,pepsin addition 4%,acetic acid concentration 0.50 mol/L,material to liquid ratio 1:15 g/mL.Under these conditions,the extraction rate was 30.33%±0.19%. Ultraviolet spectrum and twelve alkyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE)analysis showed that the collagen protein was conformed to the collagen type I characteristics and maintained three helix structure of collagen protein. Compared with the collagen of bovine hoof,camel paw collagen had better hygroscopicity than cow hoof collagen,and moisture retention was weaker than bovine hoof collagen.