Analysis of Microbial Diversity and Functional Prediction of Douzhir Based on 16S rDNA High-throughput Sequencing Technology

YUAN Yu; LI Jing; LIN Shao-hua; JIA Hong-liang; PAN Yan; LUO Hong-xia; DENG Mao-cheng

doi:10.13386/j.issn1002-0306.2020.02.016

YUAN Yu, LI Jing, LIN Shao-hua, JIA Hong-liang, PAN Yan, LUO Hong-xia, DENG Mao-cheng. Analysis of Microbial Diversity and Functional Prediction of Douzhir Based on 16S rDNA High-throughput Sequencing Technology[J]. Science and Technology of Food Industry, 2020, 41(2): 95-100. DOI: 10.13386/j.issn1002-0306.2020.02.016

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Analysis of Microbial Diversity and Functional Prediction of Douzhir Based on 16S rDNA High-throughput Sequencing Technology

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The dominant bacteria in Douzhir were studied by 16S rDNA sequencing in six representative Douzhir shops in different urban areas of Beijing. The results showed that Lactobacillus was the dominant bacteria in Douzhir,and the proportion of Lactobacillales was more than 95.8%. The dominant strains were Weissella cibaria and Lactobacillus sakei. The function of Lactobacillales in Douzhir was predicted and analyzed. It was speculated that the flavor substances in Douzhir originated from amino acid metabolism. The production of beta-galacto-oligosaccharides and GOS during fermentation was beneficial to the health of intestinal flora.

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Analysis of Microbial Diversity and Functional Prediction of Douzhir Based on 16S rDNA High-throughput Sequencing Technology

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