Study on Determination of Apigenin and Other Five Components in Propolis Health Foods by Quantitative Analysis of Multi-components by Single-maker

Objective:To establish the "quantitative analysis of multi-components by single-marker(QAMS)"method for the determination of apigenin,kaempferol,chrysin,pinocembrin and galangin in health food with propolis as a single raw material. Methods:High performance liquid chromatography was used and apigenin as reference substance to establish its relative correction factors with kaempferol,chrysin,pinocembrin and galangin. Using relative correction factor to calculate the content of five components to realize"QAMS". At the same time,the method of standard curve was used to compare the results and verify the feasibility of the method. Results:Within a certain linear range,5 components had good linear relationships,r>0.9999.The average recovery rate of 5 components were in the range of 96.54%~102.06%,and the relative standard deviations(RSDs)were in the range of 1.4%~2.8%. The relative correction factors(RCFs)of kaempferol,chrysin,pinocembrin and galangin with reference to isoferulic acid were 1.1929,0.5938,2.7847,0.9727,respectively. The relative correction factors of each component had good reproducibility under different experimental conditions. There was almost no difference between the calculated values of "QAMS" method and the measured values of standard curve method and the relative average deviations were in the range of 0.5%~2.1%. Conclusion:The method of "QAMS" is simple and reproducible,and can provide reference for the quality evaluation of health food with propolis as a single raw material.