Antibacterial Activity Evaluation of Bupleurum chinensis from Different Habitats Based on Biothermodynamics

Objective:The purpose of this study is to investigate the quality of Bupleurum chinensis from different habitats and provide theoretical basis for antibacterial active ingredient of Bupleurum chinensis. Methods:The contents of saikosaponin a and saikosaponin d in Bupleurum from different habitats were determined by high-performance liquid chromatography(HPLC)method. The growth thremogram curves of Staphylococcus aureus treated with Bupleurum from different habitats were determined by microcalorimetry,and the biothermodynamics parameters in the curve were performed by correlation analysis. Results:The results showed different about thermodynamic parameters among the difference Bupleurum area,the inhibition rate changed between 7.14% and 53.06%,which indicated that there was different degrees of inhibitory effects on the growth of Staphylococcus aureus,and Bupleurum from shanxi and shannxi showed stronger bacteriostatic activity than hebei. The highest content of saikosaponin in Bupleurum from Shanxi S3 has the strongest antibacterial effect. Spearman bivariate correlation analysis was employed to investigate the relationship between the peak areas and the inhibition by SPSS software,the chromatographic peaks with correlation coefficient greater than 0.5 were identified,the correlation coefficients of saikosaponins a and d were 0.783 and 0.717,which indicated that there was a close relation between saikosaponins a and saikosaponins d in Bupleurum and the antibacterial activity of Bupleurum chinense. Conclusion:Microcalorimetry can detect the antibacterial effect of Bupleurum chinensis from different habitats quickly and accurately. Chemical composition detection combined with biothermodynamic can be used to evaluate the biogical quality of Bupleurum chinensis.