Antiseptic Effect of <i>Pseudomonas</i> Bacteriophages PrH-181 on Large Yellow Croaker Meat

HU Shencai; CHU Lele; ZHOU Min

doi:10.13386/j.issn1002-0306.2019110135

Volume 42 Issue 6

Mar. 2021

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Science and Technology of Food Industry > 2021 > 42(6): 288-291,324. > DOI: 10.13386/j.issn1002-0306.2019110135

HU Shencai, CHU Lele, ZHOU Min. Antiseptic Effect of Pseudomonas Bacteriophages PrH-181 on Large Yellow Croaker Meat[J]. Science and Technology of Food Industry, 2021, 42(6): 288-291,324. DOI: 10.13386/j.issn1002-0306.2019110135

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Antiseptic Effect of Pseudomonas Bacteriophages PrH-181 on Large Yellow Croaker Meat

1. School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China;
2. Hubei Province Fresh Food Engineering Research Center, Wuhan 430023, China

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Received Date: November 13, 2019
Available Online: March 15, 2021

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Abstract

Abstract

In order to control the spoilage of raw large yellow croaker fillets caused by Pseudomonas. The antibacterial ability of the Pseudomonas bacteriophage PrH-181 against the spoilage of large yellow croaker under aerobic refrigeration at 4℃ was studied. After treating the fish juice with different titer of 10² to 10⁸ pfu/mL, incubating for 14 h at 30℃, the results of OD_{620 nm}showed the bacteriophage PrH-181 exhibited significant(P<0.05) antibacterial activity, when its titer was greater than 10³ pfu/mL.From the change of colony numbers, pH and TVB-N concentration which characterize the extent of spoilage, as for the control, there were 10⁶ cfu/g Pseudomonas, which was the critical value as symbolic of spoilage beginning, in the fourth day, and the minimun pH occurred in the second day, and the TVB-N concentration exceeded the critical value 30 mg/100 g on the fourth day. As for 10⁴ pfu/mL and 10⁶ pfu/mL phage-treated group, the time for the critical value of colony numbers occurred on the 10th and 12th day, and minimum pH occurred on the 8th and 12th day, TVB-N concentration was up to the critical value on the 12th and 14th day. So, it could be inferred that the preservation effect of bacteriophage PrH-181 on the large yellow croaker was obvious, and the higher the titer was, the better the preservation effect would be.
- large yellow croaker,
- antisepsis control,
- Pseudomonas,
- Pseudomonas bacteriophage PrH-181

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