Screening of High Antioxidant Activity Lactic Acid Bacteria in Traditional Fermented Pickles and Its Tolerance to Simulated Gastrointestinal Environments

Traditional fermented pickles were used as raw material,and the strains with high antioxidant activity were obtained by primary screening of hydrogen peroxide method,re-screening based on free radical scavenging rate and reducing capacity. The tolerance to the simulation environment based on gastrointestinal tract was determined. And the selected strains were identified by the sequence of 16S rDNA gene. The results showed that:14 LABs(Lactic acid bacteria strains)among 75 strains with antioxidant activities had higher DPPH radical scavenging activity(DPPH radical scavenging rate>30%). Finally,2 LABs(Kc 6 and Kc 13)were screened by monitoring the activity of OH· radical scavenging activity,reduction activation and ferrous ion reducing ability. The viability of the 2 LABs in acid,bile salt,artificial simulation of gastric and intestinal juice tests exceeded 60%. The 2 LABs were both identified as Lactobacillus plantarum. Lactic acid bacteria with both antioxidant activity and environmental tolerance could provide new ideas for the development of functional lactic acid bacteria resources with colonization strain in vivo.