Optimization of Extraction Technology of Flavonoids, Proanthocyanidins and Polyphenols from Cabernet Sauvignon Stalk

The extraction technology of flavonoids,proanthocyanidins(PC)and polyphenols from cabernet sauvignon stalk were studied. The contents of flavonoids,PC and polyphenols as indexes for comprehensive score,the orthogonal design was used to optimize the ultrasonic assisted aqueous two-phase(UATP)extraction conditions of flavonoids,PC and polyphenols from cabernet sauvignon stalk. The results showed that the optimum conditions of UATP extraction were solid-liquid ratio 1:40 (g/mL),the ultrasonic time 50 min,the ethanol mass fraction 24%,the mass fraction of ammonium sulfate 20%. Under the optimum extraction conditions,the process was magnified eight times,contents of flavonoids,polyphenols and PC in cabernet sauvignon stalk were 72.85,122.21,87.67 mg/g,respectively,the relative standard deviation was less than 3%,which indicated that the process was feasible.