Purification of Longan Seeds Polyphenols by Macroporous Resin and Its Component Analysis

In order to isolate and purify polyphenols of longan seeds,effects of four macroporous resins,the concentration and pH of ethanol on the static adsorption and desorption of longan seeds polyphenols were investigated. Main components in the extracted and purified material were identified by HPLC-Q-TOP-MS/MS. The results showed that AB-8 was the ideal resin for the purification of longan seeds polyphenols. Its static adsorption and desorption rates were 51.36% and 96.40%. The ethanol concentration and pH value with the best static desorption effect were 70% and 4,respectively. 13 major chemical constituents were identified by HPLC-Q-TOP-MS/MS in the extraction,among which 8 components were identified in longan seeds for the first time.