Effects of Theabrowins Extracted from Pu'er Tea on Key Enzymes and Tissue Sections of Glycolipid Metabolismand with High Sugar Diet Rats

Objective:Effect of the abrownin (TB) extracted from Pu'er tea on glucose and lipid metabolism index and pathology section of livers and pancreas in rats with high sugar diet was explored.Methods:Sixty male Sprague Dawley rats,according to their body weight,serum total cholesterol,serum triglycerides,high-density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) and fasting blood glucose,there was no significant difference was divided into 6 groups (n=10):normal control group,high sugar model group,positive (rosiglitazone) control group,and low-,medium-,and high-doseTB treatment groups (0.281,0.562 and 1.124 g/kg body weight,respectively). The experimental rats were given daily for free feeding and drinking. The normal control group was fed with basic feed and distilled water,and the other groups were fed with high sugar feed and 15% fructose water for 9 weeks. The serum glucose and lipid metabolism index was measured,and the pathological changes of rat liver and pancreas were observed by electron microscopy.Results:After the stage of intervention,compared with the high glucose diet group,TB could inhibit the increase of FINS,HOMA-IR,LPL,SCD-1,FAS,TNF-α,IL-1β,IL-6 (p<0.05) levels in the serum of hyperglycemic diet rats and decrease HOMA-β,HSL,FFA and LEP levels.Simultaneously,TB could significant increase the activity of GK,HMG-COA,HOMA-IS (p<0.05),and increase PK,FACL levels.Observing the liver and pancreas of rats with the microscope,lesions of high sugar diet group could be improved by intragastricTB. It indicated that TB could reduce lipid deposition in the liver and the degree of islet loss in rats on the high sugar diet,and prevent hyperglycemia. Conclusion:TB could regulate the key enzyme activities of glucose and lipid metabolism,accelerate lipid metabolism and reduce the degree of islet loss in rats with high glucose diet.