Extraction,Purification and Structural Characterization of <i>β</i>-Glucosidase in Jincheng Oranges

REN Jing-nan; FAN Gang; ZHANG Lu-lu; PAN Si-yi; WANG Ke-xing

doi:10.13386/j.issn1002-0306.2019.15.014

REN Jing-nan, FAN Gang, ZHANG Lu-lu, PAN Si-yi, WANG Ke-xing. Extraction,Purification and Structural Characterization of β-Glucosidase in Jincheng Oranges[J]. Science and Technology of Food Industry, 2019, 40(15): 84-88. DOI: 10.13386/j.issn1002-0306.2019.15.014

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Extraction,Purification and Structural Characterization of β-Glucosidase in Jincheng Oranges

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In this paper,the β-glucosidase in peel and pulp of Jincheng oranges was extracted and purified. The results showed that the activity of β-glucosidase in 0.35 mol/L NaCl eluent from peel was (6.57±4.27)×10^-3 U/g,which was significantly higher than that in 0.4 mol/L NaCl eluent,and it was also higher than that in 0.35 mol/L and 0.4 mol/L NaCl eluent from pulp (p<0.05). The protein content in 0.35 mol/L NaCl eluent from peel was also the highest at the level of 0.154 mg/mL. The molecular weight of this β-glucosidase was higher than 170 kDa,and the maximum fluorescence absorption was at 582 nm,and the size was about 5~25 nm.

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Extraction,Purification and Structural Characterization of β-Glucosidase in Jincheng Oranges

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