Establishment of Rapid Determination of Total Flavonoids in Shanxi Aged Vinegar by Raman Spectroscopy

To establish a Raman rapid detection method for total flavonoids in Shanxi aged vinegar. Crude extract of total flavonoids from Shanxi aged vinegar was obtained by ethanol-ultrasonic extraction, then the crude extract of total flavonoids was further purified. The total flavonoids was detected by a portable Raman instrument.Then the detection conditions were optimized, including integration time, laser power and number of integrations. The results indicated that the Raman characteristic peak of total flavonoids was at 731 cm^-1. The best detection condition were integration time 25 s;laser power 300 mW;the number of integration 5 times. The relative standard deviation (RSD) values of precision, reproducibility and stability were 7.82%, 4.33%, 4.11%, and the average spike recovery was 98.75%. The newly established method for rapid detection of total flavonoids in Shanxi aged vinegar meets the requirements of on-site inspection.