Optimization of the Extraction of Chlorogenic Acid from Honeysuckle with Ultra-high Pressure Treatment by Response Surface Methodology

Honeysuckle chlorogenic acid was extracted from honeysuckle by adding ethanol after ultra-high pressure treatment. After concentration and purification, the chlorogenic acid extract of honeysuckle was obtained. The extraction conditions (extraction pressure, holding time, extraction time, liquid/solid ratio and ethanol concentration) were studied according to the combination of single factor experiment and response surface methodology (RSM).The optimal extraction condition was as follows:the pressure was 325 MPa, the holding time was 10 min, the extraction time was 2 h, the liquid/solid ratio was 1:10 g/mL, ethanol concentration was 60%, with an extraction rate of 4.872%±0.049%. It was showed that ultra-high pressure treatment could increase the yield of honeysuckle chlorogenic acid.