JI Xiu-feng, LV Chang-xin, LU Yu, MA Le, YU Yong-bo, WANG Wei-min, WANG Gui-hong, LI Jian-rong. Response Surface Optimization of Alkaline Degradation Process of High Polymeric Proanthocyanidins from Red Raspberry Seed and Inhibitory Activities on Hypoglycemic Enzymes[J]. Science and Technology of Food Industry, 2019, 40(4): 159-165. DOI: 10.13386/j.issn1002-0306.2019.04.026
Citation:
JI Xiu-feng, LV Chang-xin, LU Yu, MA Le, YU Yong-bo, WANG Wei-min, WANG Gui-hong, LI Jian-rong. Response Surface Optimization of Alkaline Degradation Process of High Polymeric Proanthocyanidins from Red Raspberry Seed and Inhibitory Activities on Hypoglycemic Enzymes[J]. Science and Technology of Food Industry, 2019, 40(4): 159-165. DOI: 10.13386/j.issn1002-0306.2019.04.026
JI Xiu-feng, LV Chang-xin, LU Yu, MA Le, YU Yong-bo, WANG Wei-min, WANG Gui-hong, LI Jian-rong. Response Surface Optimization of Alkaline Degradation Process of High Polymeric Proanthocyanidins from Red Raspberry Seed and Inhibitory Activities on Hypoglycemic Enzymes[J]. Science and Technology of Food Industry, 2019, 40(4): 159-165. DOI: 10.13386/j.issn1002-0306.2019.04.026
Citation:
JI Xiu-feng, LV Chang-xin, LU Yu, MA Le, YU Yong-bo, WANG Wei-min, WANG Gui-hong, LI Jian-rong. Response Surface Optimization of Alkaline Degradation Process of High Polymeric Proanthocyanidins from Red Raspberry Seed and Inhibitory Activities on Hypoglycemic Enzymes[J]. Science and Technology of Food Industry, 2019, 40(4): 159-165. DOI: 10.13386/j.issn1002-0306.2019.04.026
Response Surface Optimization of Alkaline Degradation Process of High Polymeric Proanthocyanidins from Red Raspberry Seed and Inhibitory Activities on Hypoglycemic Enzymes
1. College of Food Science and Technology, Bohai University, Food Safety Key Lab of Liaoning Province, National&Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou 121013, China;
The alkaline degradation on red raspberry seed high polymeric proanthocyanidins was optimized by response surface methodology The hypoglycemic enzyme inhibitory ability of high polymeric polyproanthocyanidins and oligomeric procyanidins were evaluated in this research. Based on single-factor test, average polymerization degree of proanthocyanidins was taken as index. Alkaline degradation conditions of high polymeric proanthocyanidins were optimized by Box-Behnken of response surface experiment. And the inhibition effect on α-glucosidase and α-amylase ability of high polymeric polyproanthocyanidins and oligomeric procyanidins were evaluated while inhibition rate was taken as index. The results showed that alkali concentration 2.13%, solid-liquid ratio 1:10.25 (mg/mL), Degradation time 42 min, degradation temperature 60℃ made the average polymerization degree of proanthocyanidins dropped from 5.44 to 2.14±0.11.The IC50 values of α-glucosidase for high polymeric polyproanthocyanidins and oligomeric procyanidins were 0.730 and 0.291 mg/mL, and their IC50 for α-amylase were 0.578 and 0.342 mg/mL, respectively. The degradation products of high polymeric proanthocyanidins showed a good inhibitory effect on α-glucosidase and α-amylase. And the activity was higher than that of high polymeric polyproanthocyanidins. Thus oligomeric proanthocyanidins have good hypoglycemic activity and are a natural herb with potential hypoglycemic activities.