Optimised Formulation of Puerarin Albumin Microsphere by Uniform Design

To optimize the formulation of puerarin albumin microspheres. The microspheres were prepared by emulsion-heat stabilization techniques using albumin as carrier. The encapsulation efficiency and drug loading rate of the puerarin albumin microspheres were selected as comprehensive evaluation indexes. The amount of bovine serum albumin, the amount of puerarin and the amount of span-80 were selected as factors.Uniform design method were used to optimize the formulation process of puerarin albumin microspheres. Optimum formulation of puerarin albumin microspheres was as follows:Bovine serum albumin was 0.20 g, puerarin was 30.00 mg, span-80 was 0.80 mL. The entrapment efficiency of optimum formulation was 76.75%±2.09% and the drug loading was 9.81%±0.14%. The formulation process of the puerarin albumin microspheres optimized by uniform design method was effective and feasible with a good prediction, and the results showed a high comprehensive score.