Optimization of Process of Collagen Oligopeptide Calcium-Chelating of Takifugu rubripes Skin

To improve the utilization and added value of the byproducts of the Takifugu rubripes. Skin of the Takifugu rubripe was utilized for preparation of collagen oligopeptide. The conditions of collagen oligopeptide chelating calcium chloride were optimized through single factor experiment and response surface methodology with chelating rate as an index. Structure characteristics of freeze-dried sample of collagen oligopeptide chelated calcium were analyzed by Ultraviolet Spectrum (UV), Flourier Transform Infrared Spectrum (FT-IR). The results showed that the optimum chelating conditions were pH9.18, the ratio of peptide to calcium was 20.55:1, the reaction temperature was 45.3℃, under this conditions, the chelating rate was up to 91.04%.Through the UV absorption and infrared spectroscopy analysis of the collagen oligopeptide and calcium-chelating small peptide, it was showed that the collagen oligopeptide reacted with calcium to produce collagen oligopeptide calcium-chelating, and a new type of product with calcium supplementation was prepared.