Quantiative Analysis Multi-components by Single-marker for the Determination of 5 Non-Volatile in Edible Roses

Objective:To establish a quantitative analysis of multi-components by single-marker(QAMS)for determination of five bioactive components(chlorogenic acid,hyperoside,apigenin-7-O-β-D-glucopyranoside,quercetin)in edible roses with rutin as the internal reference substance,and to validate the feasibility of this method. Methods:The QAMS for edible roses was established and validated and naringin was selected as the internal reference substance.The erelative correction factors(RCFs)of other five bioactive components were calculated. The contents of five components were determined by both external standard method and QAMS.The validity of the QAMS method was evaluated by comparison of the quantitative results of both methods and to validate the durability of this method. Results:There is a good linear relationship between the 5 chemical components within the corresponding linear range,with correlation coefficients higher than 0.9999,The average recovery rate of 5 components were in the range of 98.08%~99.51%,and the relative standard deviations(RSDs)were in the range of 0.91%~1.87%,RCFs of chlorogenic acid,hyperoside,apigenin-7-O-β-D-glucopyranoside,quercetin with reference to rutin were 1.910,0.728,0.698,0.382 respectively,and repeatability was good in different experimental conditions(RSD<2.0%).The quantitative results of both externalstandard method and QAMS for the five components in 6 batches of edible roses were basically identical(RSD<1.0%). Conclusion:The QAMS method is simple,accurate and can be used as a new model for multi-componentquantitation of rose and it is suitable for comprehensive quality control of edible roses.