Surface Properties and Adhesion Ability of 15 Lactic Acid Bacterias

In order to preliminarily explore the adhesion mechanism of Lactobacillus,15 lactic acid bacterias were tested for their surface hydrophobicity,self-aggregation ability and in vitro adhesion to caco-2 cells,and high-adhesion lactobacilli strains were screened out. The cell surface hydrophobicity,autoaggregation ability,and in vitro adhesion to Caco-2 cells of 15 different lactic acid bacterias were assessed to screen the highly adhesive strains. To analyze the major adhesins affecting adhesion,the adhesive ability of Lactobacillus acidophilus KLDS 1.0901 which treated with chemicals and enzymes to the Caco-2 cells was investigated by fluorescence labeling with CFDA-SE. The results revealed that Lactobacillus acidophilus KLDS1.0901 showed the highest adhesion rate to Caco-2 cells,which was 12.73%.Significant differences in hydrophobicity,autoaggregation and adhesion to Caco-2 cells were observed among the different lactic acid bacteria strains. It showed that NaIO₄,phenol,pepsin,trypsin,especially LiCl and heat treatment could significantly decrease the adhesive ability of KLDS1.0901,which indicated that the multiple adhesins of KLDS 1.0901 to Caco-2 cells might be the result of the interaction of various adhesins mainly composed of surface proteins.