Rapid Detection of AFB<sub>1</sub> by Immunochromatographic Assays based on Fluorescent Microspheres

CHENG Min; ZHANG Kai-yi; TAN Li-rong; XIA Jun; LAI Wei-hua

doi:10.13386/j.issn1002-0306.2018.23.047

CHENG Min, ZHANG Kai-yi, TAN Li-rong, XIA Jun, LAI Wei-hua. Rapid Detection of AFB1 by Immunochromatographic Assays based on Fluorescent Microspheres[J]. Science and Technology of Food Industry, 2018, 39(23): 272-275,281. DOI: 10.13386/j.issn1002-0306.2018.23.047

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Rapid Detection of AFB₁ by Immunochromatographic Assays based on Fluorescent Microspheres

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In this study,fluorescent microsphere nanoparticles were used as immune label to develop fluorescent microspheres immunochromatographic quantitative assay for detection of aflatoxin B₁ in Semen cassiae. The results showed that the linear range and the sensitivity of the fluorescent microspheres immunochromatographic quantitative assay was 0.1~2.0 and 0.034 ng/mL,respectively. The detection methods had the advantages of rapidity and simplicity,could meet the rapid and quantitative need of the on-site screening process.

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Rapid Detection of AFB1 by Immunochromatographic Assays based on Fluorescent Microspheres

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Rapid Detection of AFB₁ by Immunochromatographic Assays based on Fluorescent Microspheres