Optimization of Extraction Process of Bond Polyphenols Compounds from Adlay Seed (Coix lachryma-jobi L. var. ma-yuen Stapf) by Response Surface Methodology

In this study,adlay seed was used as the raw materials,and based on the single factor experiments,the response surface method(RSM)was employed to optimize the extraction conditions of bond polyphenols. The degrading concentrations of NaOH,time,and extracting temperature on ethyl acetate extraction were taken as single factors,and combined with bond polyphenols extraction rate as index,to research the influence between the interaction of various factors and the extraction rate of bond polyphenols in adlay. The results showed that the optimum conditions for extraction NaOH concentration was 3.26 mol/L,the degradation time was 4.6 h,and the extraction temperature of ethyl acetate was 53℃. Under this condition,the bond polyphenols extraction rate was(30.16±1.08)mgGAE/100 g,similar to the model predicted value 29.3237 mgGAE/100 g. It showed that the method of bound polyphenol extraction condition of coix seed was reliable,and had a high extraction efficiency.