Optimization of Extraction Conditions of Anthocyanin from Blueberry Pomace and Its Antioxidant Activity

Using blueberry dried pomace as raw material,acidified ethanol as solvent,extracting anthocyanin by ultrasonic assisted method,and determining its content by pH differential method,and the optimizing extraction conditions of anthocyanin from blueberry dried pomace were determined by single factor test and Box-Behnken design. And the antioxidant activity of blueberry dried pomace and wet pomace with the same dry weight were compared by ultrasound-assisted extraction and no ultrasonic assisted extraction. The results showed that,the optimized extraction process of blueberry dried pomace were liquid-solid ratio of 31 mL/g,aethyl-alcohol concentration of 63%,pH of 2.6,ultrasonic power of 500 W,and sonication time of 20 min.Under this condition,the anthocyanin content in the blueberry pomace extract was 498.84 mg/100 g. With the same raw materials,the contests of total polyphenols and anthocyanins in the extract by ulttasonic assistred was more than the process without ultrasonic assisted. With the same process conditions,the contents of polyphenols and anthocyanins of wet pomace on the same dry weight were lower than that of dried pomace,but had a higher antioxidant activity.