Ultrasonic-assisted Aqueous Two-phase Extraction of Naringin from Pomelo Peel by Orthogonal Experimental Design

An ultrasonic-assisted technology combined with 1-propanol and(NH₄)₂SO₄ aqueous two-phase system was developed for the extraction and purification of naringin from the peel of chrysanthemum core pomelo. According to the literature and preliminary studies,the influence factors of 1-propanol mass fraction,(NH₄)₂SO₄ mass fraction,sample loading,pH,ultrasonication temperature and time were investigated by single factor designs. Subsequently,the impact of various factors to optimize the process were analyzed by orthogonal experiment. Furthermore,scale-up experiment and comparison test were also been assessed. Results showed that:Naringin was mainly extracted into the top phase,and the optimal experimental conditions of 10.0 g 1-propanol/salt aqueous two-phase system,1-propanol mass fraction of 21%(w/w),(NH₄)₂SO₄ mass fraction of 18%(w/w),sample loading at 0.15 g,pH at 4.5,temperature at 40℃,time at 30 min. Under the optimal condition,the maximum yield of naringin was 4.27%,RSD was 1.23%. The 100 times scale-up results showed that the yield was 4.25%. The comparison results indicated that ultrasonic-assisted aqueous two-phase extraction was more efficient than heat reflux extraction,which had the shorter extraction time,the lower extraction temperature and the higher extraction efficiency. The ultrasonic-assisted aqueous two-phase method was suitable for the separation and enrichment of naringin from peel of chrysanthemum core pomelo. And it could provide a new way for the preparation of naringin in industrial field.