Optimization of Hot Reflux Extraction Process of Lignans in Cinnamomum camphora Leaves by Response Surface Methodology

Response surface analysis was proposed to optimize the heat reflux extraction process of lignans fromthe leaves of Cinnamomum camphora. The effects of ethanol concentration,solid-liquid ratio,time,reflux times and temperature on lignans yield were investigated by single factor experiments. Based on the single factor level determination,the Box-Behnken center combination method was applied to determine the optimization of the extraction. Results showed that the optimum extraction condition were as follows:Ethanol concentration 87%,solid-liquid ratio 1:11 g/mL,reflux times 3,the temperature 70℃. Under the optimizatiom conditions,the experiment yield of lignans was(43.39±4.91) mg/g,which had less cleviation with the predicted value(44.27 mg/g). The results demonstrated that it was practicable to optimize the heat reflux extraction process using response surface methodology.