Effects of Different Isopentenyl Diphosphate Isomerase (IDI) and 1-Deoxyxylulose-5-phosphate Synthase (DXS) on Lycopene Production in Engineering <i>Escherichia coli</i> Strains

YANG Fan; SU Bo-li; YAO Qing; LI Hua-ping; ZHU Hong-hui

doi:10.13386/j.issn1002-0306.2018.18.024

YANG Fan, SU Bo-li, YAO Qing, LI Hua-ping, ZHU Hong-hui. Effects of Different Isopentenyl Diphosphate Isomerase (IDI) and 1-Deoxyxylulose-5-phosphate Synthase (DXS) on Lycopene Production in Engineering Escherichia coli Strains[J]. Science and Technology of Food Industry, 2018, 39(18): 131-136. DOI: 10.13386/j.issn1002-0306.2018.18.024

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Effects of Different Isopentenyl Diphosphate Isomerase (IDI) and 1-Deoxyxylulose-5-phosphate Synthase (DXS) on Lycopene Production in Engineering Escherichia coli Strains

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In order to obtain more relevant functional gene resources of isopentenyl diphosphate isomerase (IDI) and 1-deoxyxylulose-5-phosphate synthase (DXS), four IDI and DXS genes were cloned from different species, including Escherichia coli, Bacillus subtilis, Myxococcus stipitatus and Deinococcus gobiensis were heterologous expressed in Escherichia coli with pTrc99aEBI. Results showed that, MyxoIDI and MyxoDXS were the most efficient species for lycopene production and the yield of lycopene reached to 10.86, 7.94 mg/g DCW. The yield of lycopene reached 15.26 mg/g DCW by co-expression of the codon optimized IDI、DXS. This study described new genetic resources for lycopene production by comparing the different IDI and DXS.

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Science and Technology of Food Industry

Effects of Different Isopentenyl Diphosphate Isomerase (IDI) and 1-Deoxyxylulose-5-phosphate Synthase (DXS) on Lycopene Production in Engineering Escherichia coli Strains

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