Simultaneous Determination of Eight Bases and Nucleosides Contents in Allium macrostemon Bunge.by HPLC

Objective:To establish a HPLC analytical method on the simultaneous determination of eight bases and nucleosides(uracil,adenine,guanine,uridine,guanosine,thymidine,adenosine and deoxyadenosine 2'-deoxyadenosine)in different parts of Allium macrostemon Bunge from different producing areas. Methods:HPLC method was used to determine contents of 38 different parts of A. macrostemon samples,and the results of 14 bulbs were analyzed by cluster analysis and principal component analysis. Results:The eight bases and nucleosides in 38 different producing areas and parts of A. macrostemon showed a good linear relationship(r＞0.9995)within their linear ranges,with the detection limit for 10.78~37.62 ng·mL^-1,the limit of quantitation for 35.81~115.28 ng·mL^-1,the average recovery for 97.72%~100.29% and RSD less than 3%. The eight nucleosides and bases could be detected from different parts of A. macrostemon. There were obviously different mass fraction and composition structure in eight bases and nucleosides among different producing areas and parts of A. macrostemon,and some non-medicine and non-food parts were higher than the bulb. 14 bulbs from different habitats could be divided into two categories for by cluster analysis. Moreover,principal component analysis showed that comprehensive quality of bulbs produced in Wanzhou in Chongqing province was best. Conclusion:This was a fast and simple method with good reproducibility,and it was appropriate for measuring contents of the eight nucleosides. Meanwhile,the results provided theoretical basis for efficient utilization on different parts of A. macrostemon.