Growth-promoting of Black Garlic on Angiogenesis

Objective:To explore the growth-promoting on angiogenesis of black garlic with the application of transgenic zebrafish in vivo model and human umbilical vein endothelial cell in vitro model. Methods:By using the flk-1:GFP zebrafish whose blood vessel was marked by GFP,during the development of the embryo,the number of intersegmental vessels and subintestinalis vessels regulated by black garlic extract(0.2~3.2 μL/mL)with the co-application of PTK787 to inhibit the growth of blood vessels was studied. On the other hand,the proliferation of human umbilical vein endothelial cells(HUVECs)mediated by different concentrations of black garlic extract using the cell proliferation test was investigated. Results:Quantitative analysis showed that black garlic extract could significantly improve the internode and subintestinal venous disorders caused by PTK787 and increase obviously the number of intersegmental vessel and subintestinalis vessel in zebrafish embryo model in a dose-dependent manner. Specially,the modulatory effects were significant while concentration of the black garlic extract was elevated to 3.2 μL/mL. And black garlic extract could completely abolish the inhibitory effects of PTK787 on angiogenesis of intersegmental vessel and subintestinalis vessel in zebrafish. Similarly,black garlic extract was involved in the acute regulation of the growth of HUVECs,and black garlic extract could accelerate the growth of HUVECs remarkably in a dose-dependent nanner,with a notably increasing activity of HUVECs. Conclusion:These data harvested in vivo and vitro models provided significantly new evidence of that black garlic was capable of inducing angiogenesis significantly.