Simultaneous Determination of Ten Carbamate Pesticide Residues in Tea by QuEChERS-HPLC with Post-column Derivatization

Objective:To establish a method for detecting ten carbamate pesticide residues in tea by QuEChERS-HPLC with post-column derivatization. Method:The carbamat pesticide residues were extracted with acetic acetonitrile solution(0.1%)followed by adding 1 g anhydrous magnesium sulfate and 1 g anhydrous sodium acetate. After 5 mL extraction processed by PSA powder,1 mL purified solution was concentrated to near dry and dissolved by accurate 1 mL acetonitrile water solution(1+1),then detected by HPLC with post-column derivatization,quantified by external standard method. Result:The ten carbamate pesticide residues had good linearities at the rang of 0.01 to 0.5 μg/mL,the correlation coefficient were 0.9996~0.9999 and the limits of detection were 0.01~0.02 mg/kg. The average recovery rates of ten carbamate pesticide residues in 3 spiked levels ranged from 81.97% to 95.59% with the relative standard deviations(RSDs)were 1.22%~4.98%. Conclusion:The proposed method was accurate,precise,convenient and rapid,which is suitable for the determination of ten carbamate pesticide residues in tea.