Manufacture of γ-Aminobutyric Acid Using Wheat Germ

This work focus on the bio-transformation for GABA by adding glutamate and pyridoxal 5-phosphate to wheat germ,which would probably provide theoretical basis for develope the safe GABA from food sources. The single factor experiment and response surface analysis were used to optimize the condition of manufacture of γ-aminobutyric acid using wheat germ. Results showed that,the optimum condition was obtained by response surface optimization as follows:The addition of glutamic acid was 80 g/L,the pH of the reaction was 5.6,the temperature of 40℃,train number 4 hours,finally,GABA(35.42±2.19) mg/L(RSD=1.94%)can eventually be generated. Response surface methodology to manufacture of γ-aminobutyric acid using wheat germ for increasing yield was viable.