Heterologous Expression of Fusion Hydrophobin in Tremella fuciformis

In order to improve the properties of hydrophobin,and to expand the potential application of hydrophobin and the edibility of Tremella fuciformis,the Pleurotus ostreatus hydrophobin gene(hyd)was ligated with hyd without the termination codon,which was the fusion hydrophobin,named H-H,and then the expression vector pEGH-H-H was constructed. The vector was transferred into Agrobacterium tumefaciens EHA105 by electroporation,and then transformed to the yeast-like cells of T. fuciformis.After hygromycin selection and PCR detection,10 transformants were randomly selected and analyzed by Southern blot to screen multi-copy and stable genetic transformants. The quantitative real-time PCR analyzed showed that the relative expression level of transformant No.5 was 11.40-fold higher than that of the wild. Finally,we extracted the fusion hydrophobin from the transformant No.5,which proved the fusion hydrophobin can be expressed in the T. fuciformis successfully. The properties of foamability and emulsibility showed that fusion hydrophobin was better than P. ostreatus hydrophobin.