Response surface optimized extraction of flavonoids from ginseng flower and antioxidant activities

To obtain the optimal microwave-assisted extraction process of total flavonoids from ginseng flower. On the basis of single-factor test,the quadratic regression parasitism model was established through the response surface methodology. The antioxidant activity was analysized after purified with D101 macroporous resin.The results indicated that the optimal extraction conditions were microwave power of 800 W,time of 30 s,alcohol concentration of 54%,material-liquid ratio of 1 g:21 g,the maximum yield of total flavonoids was 5.624 mg/g,which results fitted well with the prediction of quadratic regression parasitism model. The good antioxidant activity had been determined by scavenging effect of flavonoids from ginseng flower on 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical(71.47%),hydroxyl free radicals(49.91%)and superoxide anion free radical(72.32%)with the IC₅₀ of 0.17,0.24,1.73 mg/mL respectively,when GFF concentration was 0.24 mg/mL. All results established a good scavenging ability to free radicals of flavonoids of ginseng flower with good development.