Process optimization of oil and hydrolyzed protein from safflower by hydrophobic enzyme

The safflower oil and hydrolyzed protein were simultaneously extracted by aqueous enzymatic method. The optimum conditions were determined by single factor and orthogonal test. The optimum conditions were determined as follows:alkali temperature was 60℃, alkali time was 60 min, and hydrolyzed by protease (1:1:1), the enzymolysis temperature was 55℃, the enzymolysis time was 3 h, the ratio of material to liquid was 1:5 (w/w), the total enzymes was 4.5%. The yield of safflower seed was 58.21%, and the yield of hydrolyzed protein was 41.32%. The optimal demulsification method was selected for the emulsion obtained after enzymolysis. The demulsification method was determined as freezing and demulsification. The centrifugal speed was 8000 r/min. The breaking rate was 35.28% and the total oil yield was 63.94%.