Effect of tuna fish bone activated calcium on viability and apoptosis of mouse embryonic osteoblast precursor cells(MC3T3-E1)

Objective:The aim of this study was to inspect the effect of citric-malic acid calcium(CMC)on the cell viability and apoptosis of mouse embryonic osteoblast precursor cells(MC3T3-E1)was investigated by using tuna bone as raw material,and to explore the mechanism of action between CMC and cells. Methods:A high concentration(1 μg/mL)of CMC and a low concentration(0.1 μg/mL)of CMC were added to the culture medium of mouse embryonic osteoblast precursor cells(MC3T3-E1),MTT assay was used to detect MC3T3-E1 cell viability,flow cytometry was used to detect serum starvation-induced apoptosis. Results:It showed that high concentration and low concentration of calcium had a significant effect on the viability of MC3T3-E1 cells. After incubation for 24 hours,the cell rate(9.67%)in the high calcium group was better than that in the low calcium group(8.95%). The proliferation rates of low calcium group were 14.96% and 20.33% at 36h and 48h,respectively,which were significantly higher than those in high calcium group(6.23% and 1.73%)after 36h and 48h. The apoptotic rate of the two groups was significantly lower than that of the blank group(p<0.01). Conclusion:CMC high calcium group and CMC low calcium group in a certain concentration range,can promote MC3T3-E1 cell viability significantly enhanced,and inhibit the serum starvation-induced osteogenic precursor cell apoptosis.