Screening of Lactobacillus casei and application in active lactic bacteria beverage

Objectives:For selecting finest probiotics can be applied to lactobacillus beverages.Methods:120 strains of Lactobacillus casei isolated from fermented foods of Yunnan provice were studied,for obtaining Lactobacillus caseis that can adapt to simulated digestive tract environment,inhabite intestinal pathogenic bacterias. Then,they are used in fermentation experiment of brown production of active lactobacillus drink.Conclusion:we screen out 4 strains of Lactobacillus casei including L.casei1,L.casei2,L.casei3,L.casei5 with strong tolerance to simulated gastric fluid,man-made digestive juice and bile salt,the four strains of Lactobacillus casei have antagonistic activity against the intestinal pathogenic bacterias,sothey have probiotic potential adjusting the balance of intestinal flora. L.casei1,L.casei2,L.casei3,L.casei5 can satisfy the production requirements through fermentation experiments of brown production of active lactobacillus drink.