Development of a mutiplex real-time PCR assay for detection of the toxin gene to <i>Bacillus cereus</i>

LIU Fang; CHEN Guo-pei; WANG Yuan-yang; GUO Zheng-yang; CHEN Jing; LIU Zhong-dong; LAN Quan-xue

doi:10.13386/j.issn1002-0306.2018.02.048

LIU Fang, CHEN Guo-pei, WANG Yuan-yang, GUO Zheng-yang, CHEN Jing, LIU Zhong-dong, LAN Quan-xue. Development of a mutiplex real-time PCR assay for detection of the toxin gene to Bacillus cereus[J]. Science and Technology of Food Industry, 2018, 39(2): 255-260. DOI: 10.13386/j.issn1002-0306.2018.02.048

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Development of a mutiplex real-time PCR assay for detection of the toxin gene to Bacillus cereus

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Objective:To develop a fast,accurate and simple method to detect the toxin genes in Bacillus cereus,and provide basis and convience for a deeper research. Methods:Three multiplex real-time fluorescence PCR systems were developed by TaqMan Probe method for the detection of toxin genes of hblA-hblC-hblD for the first PCR reaction system,nheA-nheB-nheC for the second,entFM-bceT-ces for the third. Results:The detection results of toxin genes in Bacillus cereus indicated excellent specificity and sensitivity,detection of limit of bacterial concentration was 63 CFU/mL. Conclution:The systems above had the characteristics of time saving and labor saving. They had excellent specificity and sensitivity.

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Development of a mutiplex real-time PCR assay for detection of the toxin gene to Bacillus cereus

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