Enzyme assisted ethanol extraction of luteolin from Paeonias suffruticosa pericarp

Using the method of enzyme assisted ethanol extraction of luteolin in Paeonias suffruticosa "Feng Dan" pericarp. Firstly,taking the yield of luteolin as assessment index,effects of volume fraction of ethanol,soak time,liquid to solid ratio,the extraction times were investigated,and the optimal combination was discovered by orthogonal experiment on the basis of single factor experiment. On this basis,effects of pectinase amount,water bath time,pH,water bath temperature were investigated,and the optimal combination was discovered by orthogonal experiment on the basis of single factor experiment. The optimal factors conbination was as follows:the pericarp pretreated with pectinase content of 0.05 g·L^-1,water bath time of 160 minutes,pH4.0,water bath temperature of 55℃,and then extracted in 60% ethanol for 30 min,liquid to solid ratio of 10:1,extract for 3 times,and the maximum yield of luteolin was up to 19.30 mg·g^-1.