CAI Xing-hua, REN Hao-feng, MENG Yong-shuang, ZHONG Juan, TANG Qian-yu, HUANG Jing-jie, LIN Ri-hui. Effects of low frequency electrostatic field radiation on growth and metabolism of Saccharomyces cerevisiae[J]. Science and Technology of Food Industry, 2018, 39(2): 124-127,133. DOI: 10.13386/j.issn1002-0306.2018.02.024
Citation: CAI Xing-hua, REN Hao-feng, MENG Yong-shuang, ZHONG Juan, TANG Qian-yu, HUANG Jing-jie, LIN Ri-hui. Effects of low frequency electrostatic field radiation on growth and metabolism of Saccharomyces cerevisiae[J]. Science and Technology of Food Industry, 2018, 39(2): 124-127,133. DOI: 10.13386/j.issn1002-0306.2018.02.024

Effects of low frequency electrostatic field radiation on growth and metabolism of Saccharomyces cerevisiae

  • In order to explore the effect and mechanism of low frequency electrostatic field radiation on the growth and metabolism of Saccharomyces cerevisiae,Saccharomyces cerevisiae was radiated by 60 Hz low frequency electrostatic field,and its growth curve,morphological characteristics,dextrose metabolism,total intracellular protein and the enzyme activity of alcohol dehydrogenase were investigated by scanning electron microscopy,flow cytometry,microplate reader and ultraviolet spectrophotometer in this work. Experimental results showed that low frequency electrostatic field radiation could shorten the growth delay period of Saccharomyces cerevisiae and accelerate its logarithmic growth period,its growth rate was 14.5% higher compared with the natural growth. In addition,the consumption of dextrose of the radiation growth Saccharomyces cerevisiae (LFEF treatment for 16 h)was increased by 15.64%,and the specific activity of intracellular alcohol dehydrogenase(ADH)was 7.25% higher than that of natural growth,but the total intracellular protein had no significantly different. It was indicated that the low frequency electrostatic field radiation can affect the original metabolic mechanism of Saccharomyces cerevisiae and promote its growth and metabolism process.
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