LIU Guang-xian, ZHU Shui-lan, ZHOU Jin-ying, XIONG Hui-wei, XING Sheng-ping, FU Xiao-ji, FENG Jian-xiong. Effect of epicatechin on glycation reaction of human serum albumin in physiological conditions[J]. Science and Technology of Food Industry, 2017, (24): 65-68. DOI: 10.13386/j.issn1002-0306.2017.24.013
Citation: LIU Guang-xian, ZHU Shui-lan, ZHOU Jin-ying, XIONG Hui-wei, XING Sheng-ping, FU Xiao-ji, FENG Jian-xiong. Effect of epicatechin on glycation reaction of human serum albumin in physiological conditions[J]. Science and Technology of Food Industry, 2017, (24): 65-68. DOI: 10.13386/j.issn1002-0306.2017.24.013

Effect of epicatechin on glycation reaction of human serum albumin in physiological conditions

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  • Received Date: September 13, 2017
  • In order to reveal the effect of epicatechin on protein glycosylation in physiological conditions, human serum albumin and ribose were used to construct Maillard reaction model.Inhibition rate, molecular weight, thermal aggregation, pH, free amino group content and surface hydrophobicity were analyzed in this study. The results showed that epicatechin had an inhibitory effect on glycosylation of human serum albumin, and with the increase of concentration, the inhibition rate of glycosylation increased.When the concentration of epicatechin was 0.04 mol/L, the inhibition rate was close to 80%.The results of molecular weight determination of glycosylation products showed that the molecular of glycosylation products decreased and no polymer was produced after adding the inhibitor. Analysis of the properties of glycosylation products showed that the thermostability of the glycosylation products were poor.The pH, free amino group content and surface hydrophobicity of the glycosylation products increased with the increase of epicatechin concentration, which indicated that glycosylation reaction was inhibited, the properties of the glycosylation products had changed.Therefore, epicatechin can be used as an inhibitor of glycosylation reaction in physiological conditions.
  • [1]
    袁媛, 侯雪峰, 封亮, 等.葛根素对体内外晚期糖基化终末产物形成的抑制作用[J].中草药, 2017, 48 (7) :1386-1390.
    [2]
    Zhao Y, Cai Q, Jin T, et al.Effect of Maillard reaction on the structural and immunological properties of recombinant silver carp parvalbumin[J].LWT-Food Science and Technology, 2017, 75:25-33.
    [3]
    Iwan M, Vissers Y M, Fiedorowicz E, et al.Impact of Maillard Reaction on Immunoreactivity and Allergenicity of the Hazelnut Allergen Cor a 11[J].Journal of Agricultural and Food Chemistry, 2011, 59 (13) :7163-7171.
    [4]
    Hou L, Xie J, Zhao J, et al.Roles of different initial Maillard intermediates and pathways in meat flavor formation for cysteinexylose-glycine model reaction systems[J].Food Chemistry, 2017, 232:135-144.
    [5]
    Abdelhedi O, Mora L, Jemil I, et al.Effect of ultrasound pretreatment and Maillard reaction on structure and antioxidant properties of ultrafiltrated smooth-hound viscera proteins-sucrose conjugates[J].Food Chemistry, 2017, 230:507-515.
    [6]
    刘玲, 闫凤娇, 石飞, 等.食品加工中晚期糖基化终产物的变化趋势及茶多酚对其抑制作用[J].中国食品学报, 2016 (5) :95-102.
    [7]
    朱晓琳, 刘跃钧, 陆敏, 等.不同品种马兰的活性组分及抑制蛋白糖基化功能[J].食品科学, 2014 (9) :90-95.
    [8]
    陈绍红, 刘少彬, 赵云涛.苦瓜提取物抑制蛋白质的非酶糖基化[J].中国实验方剂学杂志, 2012 (15) :211-214.
    [9]
    杨立军, 冯翠霞, 武召珍, 等.儿茶素对甘油醛白蛋白非酶糖基化的抑制作用[J].中国兽医杂志, 2015 (1) :25-27.
    [10]
    包怡红, 王文琼, 陈颖.p H对乳清蛋白糖基化产物体外抗氧化特性的影响[J].食品与发酵工业, 2011 (11) :134-140.
    [11]
    Cuevas E, Limón D, Pérez-Severiano F, et al.Antioxidant effects of Epicatechin on the hippocampal toxicity caused by Amyloid-beta 25-35 in rats[J].European Journal of Pharmacology, 2009, 616 (1-3) :122-127.
    [12]
    孙黎, 周娟, 徐自奥, 等.儿茶中表儿茶素提取工艺研究[J].安徽中医药大学学报, 2016 (2) :80-83.
    [13]
    Mendoza-Wilson A M, Glossman-Mitnik D.Theoretical study of the molecular properties and chemical reactivity of (+) -catechin and (-) -epicatechin related to their antioxidant ability[J].Journal of Molecular Structure Theochem, 2006, 761 (1-3) :97-106.
    [14]
    Due1as M, González-Manzano S, González-Paramás A, et al.Antioxidant evaluation of O-methylated metabolites of catechin, epicatechin and quercetin[J].Journal of Pharmaceutical and Biomedical Analysis, 2010, 51 (2) :443-449.
    [15]
    李晓明, 邓荣华, 孔阳辉, 等.芦丁抑制牛血清白蛋白糖基化[J].食品科学, 2014 (3) :85-89.
    [16]
    钟比真, 涂宗财, 王辉.微波场中卵清蛋白糖基化产物不均匀性的光谱分析[J].光谱学与光谱分析, 2017 (6) :1896-1901.
    [17]
    Totlani V M, Peterson D G.Reactivity of epicatechin in aqueous glycine and glucose Maillard reaction models:quenching of C2, C3, and C4 sugar fragments[J].Journal of Agricultural and Food Chemistry, 2005, 53 (10) :4130-4135.
    [18]
    常海霞, 石燕, 王辉, 等.超声波对草鱼肌肉肌原纤维蛋白溶液理化特性的影响[J].食品科学, 2015 (5) :56-60.
    [19]
    Zhang Q, Tu Z, Xiao H, et al.Influence of ultrasonic treatment on the structure and emulsifying properties of peanut protein isolate[J].Food and Bioproducts Processing, 2014, 92 (1) :30-37.
    [20]
    Sevo M, Degrassi G, Skoko N, et al.Production of glycosylated thermostable Providencia rettgeri penicillin G amidase in Pichia pastoris[J].FEMS Yeast Res, 2002, 1 (4) :271-277.
    [21]
    王松, 夏秀芳, 黄莉, 等.湿法糖基化改性对大豆分离蛋白功能性质的影响[J].食品科学, 2014, 35 (9) :38-42.
    [22]
    Xiong W, Wang Y, Zhang C, et al.High intensity ultrasound modified ovalbumin:Structure, interface and gelation properties[J].Ultrasonics Sonochemistry, 2016, 31:302-309.
    [23]
    许晶, 齐宝坤, 赵青山, 等.大豆分离蛋白结构特性与表面疏水性的关系[J].中国粮油学报, 2015 (8) :32-36.
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