Optimization of water-soluble protein extraction process of royal jelly by water extraction and acid precipitation

This experiment chose the royal jelly from Heilongjiang Mishan as raw material and took water-soluble protein extraction rate as evaluation indicator.Based on single factor experiment and Plackett-Burman design, the response surface optimization method was used to optimize the extraction of water-soluble protein from royal jelly by water extraction acid precipitation.The experiment showed that the optimum technological parameters were centrifugal speed 10000 r/min, centrifugation time 25 minutes, material water mass ratio 1 ∶ 20, ultrasonic extraction time 41 minutes, precipitation time43 minutes. Under the condition, the water-soluble protein extraction rate of royal jelly was up to 69.97%, this method was the better way to extract royal jellywater-soluble protein.