Isolation of epiphytic yeasts from the Xiahei grape and its identification by polyphasic procedure

Wild yeast strains are important resources for brewing wine.In this study, in order to find valuable wild yeast strains, streak plate separation procedures were used and three strains, YYMPT-1, YYMPT-2 and YYMPT-3, were obtained using Xiahei grape as object. They were identified as Hanseniaspora uvarum, Sporidiobolus pararoseus and Candida zemplinina by amplifying D1/D2 domain of 26 S ribosomal gene ( 26 S r DNA) , internal transcribed spacer sequence ( ITS1-5.8 S-ITS2) and actin gene by polymerase chain reaction ( PCR) , and established their phylogenetic tree, as well as observed their morphological features by scanning electron microscopy ( SEM) . The results from the current research provided 3 potential strains used for industrial applications for the production and improvement of wine quality with desirable flavor.