Study on extraction technology of polysaccharide in Camellia oleifera cake by ultrasonic cooperated with protease

Objective: To optimize the extraction process of polysaccharide from Camellia oleifera cake, it provides reference for large-scale industrial production.Method: 10 single factors experiment were carried out on the polysaccharide extraction of oil tea cake from Hunan Changde local Camellia oleifera ( Cake) varieties. Principal component analysis was used to select the 4main single factors of affecting the extraction yield of polysaccharides, and 4 factors and 3 levels orthogonal tests were carried out.Result: The optimum extraction process of polysaccharide from Camellia oleifera cake was obtained: 3.0 h enzyme reaction time, 1∶ 30 ( g/m L) material to liquid ratio, 2.0 h ultrasonic extraction time, 85 ℃ extraction temperature.Combined with the other six optimal single factor experimental conditions ( 6 month storage period, 90 mesh camellia cake powder, 3% enzyme concentration, 1000 W ultrasonic power, 3 times of extraction, 75% ethanol precipitation) , 5 parallel validation experiments were performed.The extraction yield of camellia cake polysaccharide was 7.12% ± 0.20% under this condition.Conclusion: The process is simple to operate, convenient and fast, efficiency and yields are high.It provides an effective reference standard for industrial production in the future, It provides a good reference value for the development of polysaccharide resources in Camellia oleifera cake.