Optimization of enzymatic preparation of antioxidant peptides from corn glutelin by response surface methodology

The enzymatic hydrolysis conditions of corn glutelin extracted from corn gluten meal were optimized by respones surface methodology.The DPPH radical scavenging activity was employed for the evaluation of effect in hydrolysis by Alcalase.Based on the results from single factor experiment of Alcalase, response surface methodology ( RSM) was used for the determination of the optimum conditions in the hydrolysis of corn glutelin.The optimum conditions for alcalase hydrolysis of corn glutelin were as follows: corn glutelin concentration 2%, time 2 h, p H8.0, ratio of enzyme to substrate 0.15 U/g and temperature65 ℃, and the DPPH radical scavenging activity was 69.93%. Compared with the predicted value of 70.98% by RSM, the relative difference between theoretical results and experimental results was 1.5%.