Determination of proanthocyanidins from mangosteen husk by thiolysis-HPLC and normal-phase HPLC

To investigate the content of proanthocyanidins from mangosteen husk, thiolysis-HPLC was used to analyze and quantify extractable ( EP) and nonextractable ( NEP) proanthocyanidins, then normal-phase HPLC was applied to isolate and determinate EP in different degrees of polymerization.Proanthocyanidins from mangosteen husk mainly consisted of epicatechin, contents and mean degree of polymerization of EP and NEP were ( 131.69 ± 11.24) , ( 36.92 ± 2.38) g/kg procyanidin B2/kg and ( 4.27 ± 0.08) , ( 5.41 ± 0.04) respectively.Contents of B-type procyanidin dimer, trimer and tetramer from EP were ( 40.51± 8.30) , ( 38.70 ± 8.69) , ( 13.95 ± 3.93) g/kg. These results can provide reference for the use of proanthocyanidins from mangosteen husk.